ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the toxicity of Radix Aristolochiae supplied experimental evidence of rational use of drug in clinic.</p><p><b>METHOD</b>After treatment with small dose Radix Aristolochiae, Guanxin Suhe Wan (with Radix Aristolochiae) and Guanxin Suhe Wan (without Radix Aristolochiae) in different group for a long- term, respectively, the biochemical indicator of PT, ALT, AST, ALB, ALP, Crea and BUN were detected, and the kidney, liver, stomach and urinary bladder were examined by pathologic assaying.</p><p><b>RESULT</b>In Radix Aristolochiae group and Guanxin Suhe Wan (with Radix Aristolochiae) group, all of biochemical indicator were changed significantly, and hepatonecrosis, renal tubular necrosis, gastric carcinoma and bladder carcinoma were discovered.</p><p><b>CONCLUSION</b>Radix Aristolochiae and Guanxin Suhe Wan (with Radix Aristolochiae) can damage kidney and liver, and cause gastric carcinoma and bladder carcinoma by intensive toxicity.</p>
Subject(s)
Animals , Male , Rats , Aristolochia , Chemistry , Toxicity , Drugs, Chinese Herbal , Toxicity , Kidney , Metabolism , Pathology , Liver , Metabolism , Pathology , Rats, Sprague-Dawley , Stomach Neoplasms , Urinary Bladder , Metabolism , Pathology , Urinary Bladder NeoplasmsABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of series of Muskone (the muskone includes Slender Dutchmanspipe Root, Inula Root and neither kind of Common Aucklandia Root) on the heart hemodynamics and myocardial consumption of oxygen in experimental dogs, and to explain its pharmacological action on cardiovascular system.</p><p><b>METHOD</b>Arterial blood pressure, coronary blood flow, resistance in coronary artery, total peripheral resistance, work of left artrium and oxygen consumption index of the cardiac muscles were observed in anaesthetic dogs.</p><p><b>RESULT</b>The series of Muskone decreased arterial blood pressure significantly, dilated coronary artery and peripheral arteries significantly, increased coronary blood flow, decreased resistance in coronary artery, improved the work of left artrium, the oxygen availability of cardiac muscles and the complaisance of arteries in cardiac muscles.</p>
Subject(s)
Animals , Dogs , Female , Male , Aristolochia , Chemistry , Asteraceae , Chemistry , Blood Pressure , Coronary Circulation , Cycloparaffins , Pharmacology , Drug Combinations , Heart , Physiology , Hemodynamics , Inula , Chemistry , Myocardium , Metabolism , Oxygen Consumption , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Vascular ResistanceABSTRACT
<p><b>OBJECTIVE</b>To evaluate the toxicity of Radix Aristolochiae and Radix Inulae, and to supply the toxicity experimental data that Radix Inulae supersedes Radix Aristolochiae in clinic.</p><p><b>METHOD</b>A long dose of Radix Aristolochice and Radix Inulae was given intragastrically to rats for six months, then drug withdrawal for a month. The hematology and biochemical indicators were measured, and the pathologic changes of kidney, liver, stomach and urinary bladder were examined.</p><p><b>RESULT</b>The rats of Radix Aristolochice showed serious toxic responses of renal tubule atrophy and necrosis, meanwhile, the levels of BUN, Cr and NAG were increased obviously. Hepatonecrosis, renal tubular necrosis, gastric carcinoma and bladder carcinoma were discovered with pathologic assaying. But the rats of Radix Inulae did not.</p><p><b>CONCLUSION</b>Radix Aristolochiae could damage kidney and liver, and cause gastric carcinoma and bladder carcinoma by intensive toxicity. Radix Inulae could take the place of Radix Aristolochiae to use in clinic.</p>
Subject(s)
Animals , Female , Male , Rats , Acetylglucosaminidase , Urine , Aristolochia , Chemistry , Blood Urea Nitrogen , Creatinine , Blood , Drugs, Chinese Herbal , Toxicity , Inula , Chemistry , Kidney Tubules , Pathology , Liver , Pathology , Necrosis , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Random Allocation , Rats, Sprague-Dawley , Stomach , Pathology , Stomach Neoplasms , Urinary Bladder , Pathology , Urinary Bladder NeoplasmsABSTRACT
<p><b>OBJECTIVE</b>To study the therapeutic effects of the series of Muskone (the Muskone includes Slender Dutchmanspipe Root, Tumuxiang, and not Slender Dutchmanspipe Root) on experimental myocardial infarct and pain in rats.</p><p><b>METHOD</b>Coronary artery ligation was applied for the model of myocardial infarct. Therapeutic effects were evaluated by measuring parameters of histomorphometry, blood plasm of ET, 6- keto-PGF1alpha and TXB2. Intraperitoneal injection acetic was applied for the model of ache, the frequency and eclipse period of writhing were evaluated its effect of resisting pain.</p><p><b>RESULT</b>The Muskone including Radix Aristolociae, the Muskone including Radix Inulae and the Muskone without Radix Aucklandiae all can decrease the area of myocardial infarction in rats, the level of TXB2, ET, and the frequency of writhing significantly. Also it can increase the level 6-keto-PGF1alpha, the ratio of 6-keto-PGF1alpha and TXB2. Single Radix Aristolociae or Radix Inulae only relieved pain.</p><p><b>CONCLUSION</b>The Muskone including Radix Aristolociae, the Muskone including Radix Inulae and the Muskone without Radix Aucklandiae all have significant therapeutic effect on both myocardial infarction and pain, while single Radix Aristolociae or Radix Inulae only can relieve pain.</p>
Subject(s)
Animals , Female , Male , Mice , Rats , 6-Ketoprostaglandin F1 alpha , Blood , Aristolochia , Chemistry , Cycloparaffins , Pharmacology , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Endothelins , Blood , Inula , Chemistry , Mice, Inbred ICR , Myocardial Infarction , Drug Therapy , Pain , Plants, Medicinal , Chemistry , Rats, Wistar , Thromboxane B2 , BloodABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of the series of Muskone (the Muskone includes Slender Dutchmanspipe Root, Tumuxiang, and not Slender Dutchmanspipe Root) on myocardial ischemia, myocardial infarction and hematological index in experimental canines, and to explain the pharmacological action and characteristic of its therapeutic effect on ischemic heart disease.</p><p><b>METHOD</b>The range and degree of myocardial ischemia was evaluated by epicardial electrogram mapping, and the range extent of myocardial infarction was determined by quantitate histology (N-BT staining method). Meanwhile, the changes of ET, TXB2, 6-Keto-PGF1alpha were determined to study the effects of the series of Muskone on myocardial ischemia, myocardial infarction and hematological index in experimental canines.</p><p><b>RESULT</b>The series of Muskone can improve myocardial ischemia and infarction in experimental canines, and relieve significantly the degree of myocardial ischemia (Sigma-ST) determined by epicardial electrogram mapping, decrease the range of myocardial ischemia (N-ST) determined by epicardial electrogram mapping and decrease infarction zone determined by N-BT staining method. And it has a significant inhibition on activity of ET induced by myocardial ischemia and infarction, and increases 6-Keto-PGF1alpha and 6-Keto-PGF1alpha/TXB2 induced by myocardial ischemia.</p><p><b>CONCLUSION</b>The series of Muskone has significant therapeutic effect on myocardial infarction.</p>
Subject(s)
Animals , Dogs , Female , Male , 6-Ketoprostaglandin F1 alpha , Blood , Aristolochia , Chemistry , Cycloparaffins , Pharmacology , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Endothelins , Blood , Inula , Chemistry , Myocardial Infarction , Blood , Drug Therapy , Pathology , Myocardium , Pathology , Plants, Medicinal , Chemistry , Thromboxane B2 , BloodABSTRACT
<p><b>AIM</b>To study the anticancer effect of artesunate and its mechanism.</p><p><b>METHODS</b>To observe the effect of artesunate on the growth of solid tumor and the expression of PCNA, Bcl-2 and Bax genes in mice bearing H22 solid hepatic carcinoma.</p><p><b>RESULTS</b>After administration of artesunate (ig, 300 mg.kg-1.d-1 x 7 d), growth of solid tumor was obviously inhibited, the tumor inhibitory rates were 49.1%, 48.7% and 46.6% and 46.6% in 3 experiments. The apoptosis of liver cancer cells were increased. Immunohistochemical staining showed that the number of Bcl-2 protein positive cells were decreased, but the number of Bax protein positive cells were increased. The PCNA positive cells were significantly lower than those in the control group.</p><p><b>CONCLUSION</b>Artesunate showed obvious anticancer activity on H22 hepatic carcinoma bearing mice and undergo apoptosis of liver cancer cells. The mechanism of anticancer effect of artesunate may be related to down-regulation of the expression of PCNA and Bcl-2 genes and up-regulation of the expression of Bax gene.</p>